Skip to main content
. 2013 Nov 19;8(11):e79622. doi: 10.1371/journal.pone.0079622

Figure 7. The 3′-end deletion analysis of P85–P87::GUS in transient tobacco protoplast assay.

Figure 7

(A) Schematic map of GUS constructs (number 1 to 12) developed for analyzing the regulatory elements present in the 3′-end of 1258 bp (P85–P87) promoter fragment. Construct 1 represents full-length (1258 bp) whereas successive 3′-deletion constructs #2 to 12 with 50 bp less at the 3′-end than the previous one. Constructs #2 to 12 with fragment size 1208, 1158, 1108, 1058, 1008, 958, 908, 858, 808, 758 and 708 bp promoter, respectively, as shown in diagram. (B) Transient GUS expression analysis of constructs 1 to 12 in pKYLX-80 background in tobacco protoplast. The average GUS activity ± SD was presented in the histogram of 3 independent experiments of 3 replications of each construct by taking the activity of full-length (1258 bp) promoter fragment as 100%.