Adiponectin levels affect angiogenic marker expression and perfusion in adipose tissue after long-term maintenance on high fat/high sucrose diet. a–c) Selected relevant mRNA levels in epididymal fat pad (EFP) of genes involved in hypoxic and angiogenic pathways as determined by PCR array, expressed relative to wild type and shown with upper and lower confidence intervals. d) Whole adipose tissue gene expression of d) VEGF and e) Shh was quantified by RT-PCR. Tissue perfusion was determined by quantifying number of microspheres in f) kidney, g) heart, and h) EFP. Data are shown as means ± SEM and represent apn-KO (n=9), wild type (n=11), and apn-TG (n=8) *P<0.01 vs. apn-KO and wild type; †P<0.001 vs. apn-KO and wild type.