. Author manuscript; available in PMC: 2014 Nov 1.

Published in final edited form as: Blood Rev. 2013 Aug 31;27(6):10.1016/j.blre.2013.08.001. doi: 10.1016/j.blre.2013.08.001

Table 2. Preclinical observations based on ex vivo Notch ligand-mediated expansion of T cell progenitors.

This table highlights studies in which Notch-expanded T cell progenitors were infused in vivo to improve immune reconstitution after transplantation. Additional studies not meeting these criteria are discussed in the text.

Cell source	Notch ligand source	Key Observation(s)	Reference
Mouse BM LSKs	OP9-Delta-like1 cells	Delta-like1 expands DN2 and DN3 pre-T cells Expanded pre-T cells increase thymic engraftment, bacterial clearance, and have NK-independent GVT effects	Zakrzewski et al., 2006 ⁸¹
Mouse BM LSKs	Immobilized Delta-1^ext-IgG	Expansion of DN2 pre-T cells correlates with Delta-like1 density Expanded pre-T cells engraft the thymus and produce mature T cells	Dallas et al., 2007 ⁶⁸
Mouse BM LSKs	OP9-Delta-like1 cells	Expanded pre-T cells induce GVT, but not GVHD, and have potential for anti-tumor engineering	Zakrzewski et al., 2008 ⁸²
Human UCB: CD34⁺ cells	OP9-Delta-like1 cells	Expanded pre-T cells engraft the thymus and produce mature T cells	Awong et al., 2009 ⁸³
Mouse BM LSKs	OP9-Delta-like1 cells	Expanded pre-T cells engraft extra-thymically, producing mature T cells	Holland et al., 2012 ⁸⁴

BM, bone marrow; LSK, Lin^-Sca-1⁺c-Kit⁺ hematopoietic stem and progenitor cells; GVT, graft-versus-tumor; GVHD, graft-versus-host disease; Ag, antigen; UCB, umbilical cord blood.