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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.002. doi: 10.1016/j.bbamcr.2013.07.002

Figure 5.

Figure 5

Effect of Sp1, Sp3, and Sp4 over-expression on the transcript and protein levels of NMDA receptor subunit genes. (A) Real-time PCR revealed an up-regulation of Sp1, Sp3, and Sp4 transcripts in N2a cells transfected with Sp1, Sp3, and Sp4 over-expression vectors, respectively. N = 6. (B) mRNA levels of Grin1, Grin2a, and Grin2b were increased with Sp4 over-expression but not with Sp3 or Sp1 over-expression. N = 6. (C-D) Western blots revealed an up-regulation of Sp4, Sp3, and Sp1 protein with Sp4, Sp3, and Sp1 over-expression, respectively, in N2a cells. β-actin served as a loading control and a representative blot is shown. N = 3. (E–F) Over-expression of Sp4 increased protein levels of GluN1, GluN2A, and GluN2B, whereas over-expression of Sp1 and Sp3 did not significantly change these subunit levels. β-actin served as a loading control. N = 3. (G) Primary neurons transfected with Sp1, Sp3, or Sp4 over-expression showed increases in Sp1, Sp3, and Sp4 transcripts, respectively. N = 3. (H) mRNA levels of Grin1, Grin2a, and Grin2b were increased with Sp4 over-expression but not with Sp3 or Sp1 over-expression in primary neurons. Grin2c levels did not increase with Sp1, Sp3, or Sp4 overexpression in primary neurons. N = 3. *= P < 0.01, **= P < 0.01, and ***= P < 0.001.