Fig. 1.

L-Cad11 cell aggregation. (A) Time course of Cad11-mediated cell-cell adhesion measured as a decrease in single cell number. L-vector did not exhibit significant aggregation activity. (B) Time course of aggregation inhibition by 20 μg/ml of IgG or anti-Cad11 polyclonal antibody. (C) Dose-dependence of aggregation inhibition by polyclonal anti-Cad11 antibodies. Results from the 3 h assay are shown (*, p < 0.05). (D) Screening for adhesion-blocking monoclonal antibodies. L-Cad11 cells were incubated with hybridoma supernatants and the time course of aggregation was measured. Untreated L-vector cells and L-Cad11 cells were used as negative and positive controls, respectively. mAb 1A5 and 2C7 were assayed twice to confirm that the inhibition was reproducible. Results from the 3 h aggregation assay are shown.