BI-1 modulates ER stress and autophagy by independent mechanisms. BI-1 suppresses IRE1α by forming a complex with IRE1α, which then nullifies both its endoribonuclease (XBP-1) and kinase activity (JNK). Via an IP3R-dependent mechanism, BI-1 also reduces the steady-state levels of ER Ca2+, which causes a corresponding decline in mitochondrial Ca2+ levels and reduces mitochondria bioenergetics. Reduction in ATP levels (rise in AMP) activates the intracellular energy sensor AMPK, which activates autophagy via effects on Atg1 (Ulk1/Ulk2). BI-1 has also been shown to associate with Bcl-2 to regulate Ca2+ homeostasis, which could also indirectly influence autophagy.