Figure 6. Spironolactone reduces bleomycin-induced circulating Ly6C^hi monocytosis.

A shows the gating strategies for mouse blood monocyte subsets. B and C show the dynamic profiles of Ly6C^hi and Ly6C^lo monocytes in all treatment groups, respectively. Panel D shows the representative temporal profiles of flow cytometry analysis (pseudocolor plots) of monocyte subsets in all treatment groups. Abbreviations: BLM, bleomycin; FSC, forward-scattered light; NS, normal saline; SP, spironolactone; SSC, side-scattered light. *P<0.05 vs. NS group. #P<0.05 vs. BLM and BLM + SP groups (n = 5 to 7).