Table 1. Primer sequences used in this study.
Primers | Sequences (5’-3’) | PCR products (bp) |
---|---|---|
MR | GGCTACCACAGTCTCCCTGA | 75 |
AGAACGCTCCAAGGTCTGA | ||
Col I | CATGTTCAGCTTTGTGGACCT | 94 |
GCAGCTGACTTCAGGGATGT | ||
Col III | TCCCCTGGAATCTGTGAATC | 63 |
TGAGTCGAATTGGGGAGAAT | ||
CCL2/MCP-1 | TTAAGGCATCACAGTCCGAG | 129 |
TGAATGTGAAGTTGACCCGT | ||
TGF-β1 | AAACGGAAGCGCATCGAA | 63 |
GGGACTGGCGAGCCTTAGTT | ||
IL-1β | AACGTGTGGGGGATGAATTG | 130 |
CATACTCATCAAAGCAATGT | ||
Arg-1 | AGGAGAAGGCGTTTGCTTAG | 115 |
AGGAGAAGGCGTTTGCTTAG | ||
β-actin | CTAAGGCCAACCGTGAAAAG | 104 |
ACCAGAGGCATACAGGGACA |
Abbreviations: Arg-1, arginase-1, Col I, collagen type I; Col III, collagen type III; CCL2, chemokine (C-C motif) ligand 2; MCP-1, monocyte chemoattractant protein-1; MR, mineralocorticoid receptor; TGF-β1, transforming growth factor β1; IL-1β, interleukin-1β.