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. 2013 Nov 19;8(11):e80975. doi: 10.1371/journal.pone.0080975

Figure 6. Combined inhibition of AMPA receptors and NADPH oxidase reduces descending serotonergic fiber loss after spinal cord injury.

Figure 6

(A) Diagram and spinal cord cross-section showing the location of descending serotonergic tracts. White box represents the bilateral area used for quantification. (B,C) Co-labeling of serotonergic fibers in the spinal cord with neurofilament and MAP2. (D) Quantification of serotonergic immunofluorescence at 3 mm caudal to lesion epicenter revealed significantly more serotonin reactivity below the level of the lesion following treatment of NBQX + Apocynin (n = 3-4). (E) Representative magnified images from the white box in (A) depicting descending serotonergic tracts 3 mm caudal to lesion epicenter. Scale bar = 20 µm. ***, p ≤ 0.001 compared to naïve; ##, p ≤ 0.01 compared to vehicle. Error bars indicate the standard error of the mean.