Figure 6. Cleavage of caspase 8 and PARP in HaCaT cells exposed to resveratrol, UVB or both.

HaCaT cells were treated with resveratrol (25 and 100 µM, 2 and 24 hours), UVB (10, 40, and 100 mJ/cm² alone or pretreated for 2 or 24 hours with resveratrol (25 and 100 µM) prior irradiation with UVB (10, 40, and 100 mJ/cm²). Lysates were collected 4 hours and 30′ after irradiation and resveratrol withdrawal and challenged with anti-caspase 8 and anti PARP antibodies recognizing the cleaved forms of the two proteins (panel A), or with anti-Beclin 1. Panel B reports the densitometric analysis of caspase 8 cleaved band and Beclin 1 at both 2 and 24 hours prior irradiation. The same lysates were challenged with anti γ-H2AX, recognizing the phosphorylated form of the histone (Panel D). GAPDH was used as loading control.