Figure 7. Multiple pathway analysis in HaCaT cells exposed to resveratrol, UVB: later time point (4.5 hours after resveratrol withdrawal and UVB irradiation).

HaCaT cells were treated with 25 µM resveratrol for 24 hours prior to irradiation with UVB (10, and 100 mJ/cm²). Cell lysates were collected after 4 hours and 30′ resveratrol withdrawal and UVB irradiation. Phosphorylated or non-phosphorylated protein levels were analyzed by western blot. Total cell lysates (30 µg) were challenged with antibodies recognizing the phosphorylated forms of ERK1/2, p38, p53, AKT, p70, and S6 and or the native form of p21, and p27. Antibodies recognizing the non-phosphorylate form of ERK1/2, p38, p53, AKT, p70, S6 and GADPH were used as loading control.