Figure 8. Effect of inactivation of Δamt-3 or Δskb-1 on growth of cot-1 ts.

cot-1 ts, grown in the presence of NaCl, exhibits a suppressed colonial phenotype. The colonial phenotype is further suppressed upon inactivation of amt-3 or skb-1. Strains were cultured for 72 hours at 34°C (cot-1 ts restrictive temperature) with 0.5, 0.75, 1 or 1.25 M NaCl. Colony area is presented as percent of control. * and + indicate a significant difference (p<0.05) of colony area in the Δamt-3;cot-1 or Δskb-1;cot-1 backgrounds, respectively.