Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Nov 19;8(11):e80716. doi: 10.1371/journal.pone.0080716

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Ning et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Schematic representation of the DNA fragments used in EMSAs. The numbers indicate the ends of the fragments relative to the transcriptional start site. + or − indicates that VapB10 binds to the DNA fragment or not. (B) EMSAs of VapB10 binding to the different regions of the promoter P_vapBC10. The DNA fragments P1, P2 and P3 were PCR amplified with the primer pairs P_vapBC10-E3/P_vapBC10-E4, P_vapBC10-E5/P_vapBC10-E6 and P_vapBC10-E7/P_vapBC10-E2. The labeled fragment P1 (lanes 1-5), P2 (lanes 6-10) and P3 (lanes 11-15) were incubated with increasing concentrations of VapB10 (see the Figure 5 legend for further details), respectively.