Figure 7. Stability of VapB10 and VapC10 in the presence of ClpPXP2s or Lons.

The E. coli strains BL21(DE3)(pJS653) (A), BL21(DE3)(pJS883) (B), BL21(DE3)(pJS429) (C), BL21(DE3)(pJS882) (D) and BL21(DE3)(pJS427) (E) were grown, induced and translationally stalled as described in Materials and methods. The cells treated at various points of time were subjected to Western blot analysis to monitor VapB10, VapC10, ClpP2s or Lons with the respective primary antibodies. The corresponding graph represents the percentages of the related protein amount at each time point compared to that at time zero.