Fig. 2. Detection of reactive oxygen species in A-10 cells treated with carbon black N990. A-10 cells were incubated with 100 μg/ml carbon black N990 for indicated durations (0, 15, 60, and 180 min). Then, cells were briefly washed with PBS, loaded with DCF-DA (2 μM), and were immediately viewed by fluorescence microscope. Hydrogen peroxide (H2O2, 200 μM), a positive control, was treated to cells for 30 min. Scale bar = 50 μm.
