Fig. 2. Histological features of ear tissue and TSLP hyperproduction in DNCB-stimulated mice. BALB/c mice were sensitized with DNCB at 2-day intervals for up to 31 days, painted with hydrocort cream and FOF (2 and 10%) every other day, as described in Figure legend 1. Ear thickness was measured on day 31. (B) Paraffin sections of ear tissues were stained with hematoxylin and eosin (H&E, × 200), and (C) processed for TSLP immunohistochemistry (IHC, × 200). (E) TSLP was measured in ear tissues by western blot. Data are representative of 8 mice per group. Values are mean ± S.D. (n = 8 mice per group). *P< 0.05 and ***P< 0.001 compared to mice stimulated with DNCB alone (induction group).