Supp Table 4.
Changes of cytokines in mesenteric lymph node after administration of ZEA to female Wistar rats (pg/mg protein)
| Parameter | Control | ZEA Low | ZEA Medium | ZEA High | Vac | ZEA Low With Vac | ZEA Medium With Vac | ZEA High With Vac |
|---|---|---|---|---|---|---|---|---|
| IL-1α | 15.3 ± 7.8 | 19.2 ± 4.2 | 21.4 ± 9.2 | 18.2 ± 5.9 | 19.6 ± 4.6 | 17.5 ± 1.4 | 16.5 ± 2.3 | 12.6 ± 2.4 |
| IL-2 | 4.4 ± 1.7 | 6.3 ± 1.5 | 7.1 ± 1.4 | 6.6 ± 3.1 | 6.3 ± 1.2 | 6.7 ± 1.1 | 5.0 ± 0.9 | 7.5 ± 1.5* |
| IL-4 | 0.5 ± 0.1 | 0.4 ± 0.2 | 0.9 ± 0.1 | 0.4 ± 0.4 | 0.4 ± 0.3 | 0.9 ± 0.7 | 0.4 ± 0.2 | 0.7 ± 0.4 |
| IL-6 | 53.5 ± 15.2 | 67.2 ± 21.7 | 84.2 ± 19.6 | 56.7 ± 21.9 | 67.8 ± 7.5 | 78.5 ± 18.9 | 63.7 ± 18.6 | 81.1 ± 22.3 |
| IL-10 | 4.5 ± 2.4 | 5.3 ± 1.3 | 6.7 ± 0.9 | 5.7 ± 2.4 | 5.4 ± 1.4 | 5.2 ± 1.0 | 5.2 ± 1.2 | 4.3 ± 0.8 |
| IFN-γ | 35.4 ± 10.4 | 43.3 ± 13.2 | 58.9 ± 16.1* | 41.6 ± 11.0 | 44.3 ± 4.6 | 54.5 ± 0.3 | 38.2 ± 8.7 | 54.8 ± 19.8 |
| GM-CSF | 0.8 ± 0.2 | 0.9 ± 0.1 | 1.0 ± 0.2 | 0.7 ± 0.1 | 1.0 ± 0.2 | 0.9 ± 0.1 | 0.8 ± 0.2 | 0.9 ± 0.2 |
| TNF-α | 4.6 ± 2.1 | 10.1 ± 4.3 | 11.2 ± 6.1 | 8.7 ± 2.2 | 10.7 ± 1.7 | 12.5 ± 6.0 | 15.6 ± 4.6* | 14.8 ± 5.4* |
Rats in ZEA low, medium and high groups were administered with 1mg, 5mg and 30mg ZEA/kg bw by gavage once per day for 35 days.
Vac groups were administered 2.0mL of inactivated porcine parvovirus vaccine subcutaneously on day 8 (Vac, ZEA Low with Vac, ZEA Medium with Vac and ZEA High with Vac groups). Values are mean ± SD of 5 replicas.
*: Significantly different from control at p<0.05.
+: Significantly different from Vac at p<0.05.