Fig. 1. Effects of various fractions from Carpinus tschonoskii on the protein production of MDC in IFN-γ-stimulated HaCaT human keratinocytes. (A) The Solvent fractionation of leaves of Carpinus tschonoskii. (B) Protein production of MDC was determined from the culture supernatant of cells stimulated with IFN-γ (10 ng/ml) in the presence of various fractions (T, EtOH; H, Hexane; C, chloroform; E, EtoAc; B, BuOH; W, H2O) of C. tschonoskii (50 μg/ml) for 24 hr. MDC production was determined by ELISA and was done in triplicate. (C) Cell viability was determined from the cells stimulated with IFN-γ (10 ng/ml) in the presence of various fractions of C. tschonoskii (50 μg/ml). Cell viability was analyzed by MTT assay. Error bars indicate ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001, significant when compared with IFN-γ positive control.
