Fig. 2. Effect of chloroform fraction from Carpinus tschonoskii on the mRNA and protein level of MDC in IFN-γ-stimulated HaCaT human keratinocytes. (A) Cells (3.0 × 105 cells/ml) were pre-incubated for 18 hr, and the protein production of MDC was determined from the culture supernatant of cells stimulated by IFN-γ (10 ng/ml) in the presence of chloroform fraction (12.5, 25, 50 μg/ ml) of C. tschonoskii. MDC production was determined by ELISA and was done in triplicate. Error bars indicate ± S.D. **, p < 0.01; ***, p < 0.001, significant when compared with IFN-γ positive control. (B) mRNA expression of MDC was determined from the cells stimulated with IFN-γ (10 ng/mL) in the presence of chloroform fraction (12.5, 25, 50 μg/ml) of C. tschonoskii for 18 hr.
