Table 1.
Mortality, clinical signs, gross and histopathological findings of animals exposed with prebrewed AS in the single dose toxicity study
| Groups | Male vehicle control | Prebrewed AS treated male rats (mg/kg) | Female vehicle control | Prebrewed AS treated female rats (mg/kg) | ||||
|---|---|---|---|---|---|---|---|---|
|
|
|
|||||||
| 2,000 | 1,000 | 500 | 2,000 | 1,000 | 500 | |||
|
| ||||||||
| Mortalitya) | 0/5 | 1/5 | 0/5 | 0/5 | 0/5 | 1/5 | 0/5 | 0/5 |
| Clinical signs | ||||||||
| Seizure | 0/5 | 2/5 | 0/5 | 0/5 | 0/5 | 1/5 | 0/5 | 0/5 |
| Loss of locomotionb) | 0/5 | 5/5 | 0/5 | 0/5 | 0/5 | 3/5 | 0/5 | 0/5 |
| Gross findings | ||||||||
| Lung focal congestion | 1/5 | 1/4 | 1/5 | 1/5 | 1/5 | 1/4 | 0/5 | 1/5 |
| Thymus focal congestion | 1/5 | 0/4 | 0/5 | 0/5 | 0/5 | 0/4 | 0/5 | 0/5 |
| Spleen atrophy | 1/5 | 0/4 | 1/5 | 0/5 | 1/5 | 0/4 | 0/5 | 1/5 |
| Lymph node hypertrophyc) | 1/5 | 1/4 | 2/5 | 2/5 | 2/5 | 1/4 | 3/5 | 1/5 |
| Uterus edema | 1/5 | 2/4 | 0/5 | 1/5 | ||||
| Histopathological findings | ||||||||
| Lung focal congestion | 1/5 | 1/4 | 1/5 | 1/5 | 1/5 | 1/4 | 0/5 | 1/5 |
| Tymus cDEd) | 0/5 | 0/4 | 0/5 | 0/5 | 1/5 | 0/4 | 0/5 | 0/5 |
| Spleen wDEe) | 2/5 | 1/4 | 1/5 | 0/5 | 1/5 | 0/4 | 1/5 | 1/5 |
| Liver focal inflammation | 2/5 | 1/4 | 1/5 | 0/5 | 2/5 | 2/4 | 1/5 | 2/5 |
| Lymph node HPcf) | 2/5 | 1/4 | 2/5 | 3/5 | 3/5 | 2/4 | 3/5 | 2/5 |
Values were observed animals/total observed animals.
AS = Armeniacae Semen aqueous extracts.
a)Each of one female and male rat treated with 2,000mg/kg of prebrewed AS was died, within 2 hr after end of treatment, respectively.
b)Loss of locomotion with increases of heart beat and respiration.
c)Submandibular lymph node.
d)Cortex lymphoid cell decreases.
e)White pulp lymphoid cell decreases.
f)Diffused hyperplasia of lymphoid cells.