Figure 4.

Relative expression of the catalytic alpha (α) domain of AMPK in proximal tubular cells derived from α1^-/- and α2^-/- knockout (KO) mice. The relative expression of the α1 and α2 isoforms of AMPK, as well as of the total α domain (α1 + α2), was evaluated in immunoblots of lysates from confluent mouse proximal tubular (MPT) cells derived from the kidneys of either α1^-/- or α2^-/- KO mice, or their WT controls. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown on the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.02, α1^-/- vs. WT mice, for α2 isoform expression; # p < 0.02, α2^-/- vs. WT mice, for α1 isoform expression.