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. 2013 Nov 14;13:159. doi: 10.1186/1471-230X-13-159

Figure 3.

Figure 3

Assessment of TNF-α production by human colonic epithelial cells. Cells were incubated for 24 h with 20 μg/ml of LPS, 1 mM of apocynin (Apoc), 20 μg/ml LPS + 1 mM apocynin or control without stimulation (as indicated). After incubation cell media were collected and TNF-α concentration was measured as described in Methods. *Statistically significant difference between control and LPS subgroups in the UC group. **Statistically significant difference between LPS and LPS + apocynin subgroups in the UC group. #Statistically significant difference between LPS subgroup in the UC and control groups. Statistically significant, as p <0.05.