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Karyotypes of Trachinotus goodei (a, d, g), Trachinotus carolinus (b, e, h) and Trachinotus falcatus (c, f, i). Conventional staining (a, b, c) highlighting the chromosomal pairs carrying Ag-NOR sites; C-banding (d, e, f); nucleolar organizer pairs are highlighted by staining with CMA3+/DAPI-. Dual-colorFISH (f, g, h) showing the chromosomal mapping of the 18S rDNA (green) and 5S rDNA (red) sites. Bar = 5 µm.
