Table 1. Primers used in the qRT-PCR.
| Primer | Sequence (5′→3′) |
|---|---|
|
β-actin |
F: CTGTCGAGTCGCGTCCACCC |
|
β-actin |
R: ACATGCCGGAGCCGTTGTCG |
|
HIF-1α |
F: CCCACCCCGCCTCTGGACTT |
|
HIF-1α |
R: TCGGTGCCCGCGTTGTCTTC |
|
VEGF-A |
F: ACTCGGATGCCGACACGGGA |
|
VEGF-A |
R: AAATGTGCCCCCGTGCCCTG |
|
Car9 |
F: GGTGCACCTCAGTACTGCTT |
|
Car9 |
R: ATGGGACAGCAACTGTTCGT |
|
GLUT1 |
F: ACCATCTTGGAGCTGTTCCG |
|
GLUT1 |
R: GCCTTCTCGAAGATGCTCGT |
|
NF-κB |
F: GGCCTTCCGAATTTGGCGTCCT |
|
NF-κB |
R: GAGTGCAGCTGAGCGAGCGA |
|
p53 |
F: GTGGGTCAGCGCCACACCTC |
|
p53 |
R: GGGGAGGAGCCAGGCCATCA |
|
Bax |
F: GTGAGGGCCGCACGTCCAC |
|
Bax |
R: CTCCCCGGACCCGTCCATCA |
|
Bcl2 |
F: CTGCCTTTTTGCCCCGCTGC |
|
Bcl2 |
R: GGGGTCTGGGGGCTAGGTGG |
|
Apaf1 |
F: ATGAAGATGAGGTGCTCTGCTGC |
|
Apaf1 |
R: GCTTCCCAGTCGCAGAATCCCA |
|
p21 |
F: TCCAGGAGGCCCGAGAACGG |
| p21 | R: CCTCCCAGGTCGTCACGGCT |