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. 1979 Apr;76(4):1756–1759. doi: 10.1073/pnas.76.4.1756

Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.

S Kimura, R V Lewis, L D Gerber, L Brink, M Rubinstein, S Stein, S Udenfriend
PMCID: PMC383469  PMID: 221906

Abstract

Pro-opiocortin was purified from camel pituitaries by procedures including high-performance liquid chromatography. The precursor relationship of the pure protein to the opioid peptides and to corticotropin was confirmed. Partial chemical analysis consisting of amino acid analysis and tryptic peptide mapping was carried out with the aid of sensitive fluorescence detection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böhlen P., Stein S., Stone J., Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. doi: 10.1016/0003-2697(75)90316-4. [DOI] [PubMed] [Google Scholar]
  2. Eipper B. A., Mains R. E. Analysis of the common precursor to corticotropin and endorphin. J Biol Chem. 1978 Aug 25;253(16):5732–5744. [PubMed] [Google Scholar]
  3. Gerber L. D., Stein S., Rubinstein M., Wideman J., Udenfriend S. Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site. Brain Res. 1978 Jul 28;151(1):117–126. doi: 10.1016/0006-8993(78)90954-x. [DOI] [PubMed] [Google Scholar]
  4. Li C. H., Chung D. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145–1148. doi: 10.1073/pnas.73.4.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Li C. H., Chung D., Yamashiro D., Lee C. Y. Isolation, characterization, and synthesis of a corticotropin-inhibiting peptide from human pituitary glands. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4306–4309. doi: 10.1073/pnas.75.9.4306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ling N., Burgus R., Guillemin R. Isolation, primary structure, and synthesis of alpha-endorphin and gamma-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3942–3946. doi: 10.1073/pnas.73.11.3942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nakanishi S., Inoue A., Kita T., Numa S., Chang A. C., Cohen S. N., Nunberg J., Schimke R. T. Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6021–6025. doi: 10.1073/pnas.75.12.6021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roberts J. L., Herbert E. Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4826–4830. doi: 10.1073/pnas.74.11.4826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts J. L., Phillips M., Rosa P. A., Herbert E. Steps involved in the processing of common precursor forms of adrenocorticotropin and endorphin in cultures of mouse pituitary cells. Biochemistry. 1978 Aug 22;17(17):3609–3618. doi: 10.1021/bi00610a030. [DOI] [PubMed] [Google Scholar]
  11. Rubinstein M., Stein S., Gerber L. D., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052–3055. doi: 10.1073/pnas.74.7.3052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rubinstein M., Stein S., Udenfriend S. Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1978 Feb;75(2):669–671. doi: 10.1073/pnas.75.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rubinstein M., Stein S., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969–4972. doi: 10.1073/pnas.74.11.4969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scott A. P., Ratcliffe J. G., Rees L. H., Landon J., Bennett H. P., Lowry P. J., McMartin C. Pituitary peptide. Nat New Biol. 1973 Jul 18;244(133):65–67. doi: 10.1038/newbio244065a0. [DOI] [PubMed] [Google Scholar]
  15. Stein S., Böhlen P., Stone J., Dairman W., Udenfriend S. Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973 Mar;155(1):202–212. doi: 10.1016/s0003-9861(73)80022-0. [DOI] [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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