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Induction of cell cycle arrest by cholbut SLN treatment. (A,B) PC-3 cells (1.5 × 105) were treated or not with 50–300 μM cholbut SLN for 24–72 h and cell cycle was then assessed by flow cytometry. (C,D) in these experiments, cholbut SLN was replenished every 24 h (48 h culture: 24 + 24 h; 72 h culture: 24 + 24 + 24 h) without changing the culture medium (A and C: representative cell cycle plots; B and D: quantification of cell cycle phases; n = 3).
