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Induction of cell death following treatment with cholbut SLN. PC-3 cells (1.5 × 105) were incubated with 50–300 μM cholbut SLN with replenishment, for 48 (24 + 24 h)–72 h (24 + 24 + 24 h) and cell death was then assessed by flow cytometry. Data shown are means ± SEM (n = 3). **P < 0.01, significantly different from control; one-way ANOVA and Dunnett's test.
