Table 2.
Phosphorylation of MLC1 from rat hearts subjected to I/R and perfused in the presence of high doses of ML-7 or Y-27635 or with a mixture of low doses of ML-7 and Y-27632
| Identified phosphorylation in myocardial MLC1 | ||||
|---|---|---|---|---|
| I/R heart (20/30) | ||||
| Aerobic heart | +ML-7 (5 μM) | +Y-27632 (1 μM) | +ML-7 (1 μM) + Y-27632 (0.05 μM) | |
| T69 | T69 | n/d | n/d | n/d |
| n/d | T77 or Y78 | T77 or Y78 | n/d | T77 or Y78 |
| T132 orT134 or T135 | T132 orT134 or T135 | T132 orT134 or T135 | T132 orT134 or T135 | T132 orT134 or T135 |
| n/d | T164 | n/d | n/d | n/d |
| n/d | S184 | n/d | n/d | n/d |
| n/d | Y190 | n/d | n/d | n/d |
T, threonine; Y, tyrosine; S, serine; n/d, not detected.