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. 2013 Aug 27;170(2):426–439. doi: 10.1111/bph.12292

Figure 7.

Figure 7

Effects of 11R-VIVIT and NFAT2 gene knockdown on the expression of the uPA receptor (Plaur) gene in podocytes treated with high glucose. (A)The mRNA level of uPA receptors was examined using real-time quantitative RT-PCR analysis, and GAPDH mRNA was used as an internal control. Quantitative data were calculated by 2-△△ CT. (B) The protein level of uPA receptors was analysed using Western blot analysis(n = 3). (C) Densitometry analysis of three repetitions of the experiment shown in (B). Note NG, normal glucose (5.3 mM) group; HG, high glucose group(30 mM); MA, normal glucose (5.3 mM) + mannitol(24.7 mM)group, as an osmolality control; HG+11R-VIVIT, High glucose (30 mM)+ 11R-VIVIT(100 nM, a cell permeable NFAT inhibitor) group; HG + NFAT2-siRNA, High glucose (30 mM) + NFAT2-siRNA (50 nM) group; HG + control-siRNA, High glucose (30 mM) + control-siRNA (50 nM), as a negative control group. All Values are expressed as the mean ± SEM. *P < 0.01 versus HG.