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Impaired Ca2+ binding by mutant calmodulin C-domains. A, Schematic model of the Ca2+ binding loops in the C-terminal EF-hand domains of calmodulin showing location of the mutations. B, Titration curves for Ca2+ binding to recombinant WT (black circles) and mutant calmodulins (F142L, blue triangles; D96V, grey diamonds; D130G, red squares). C, Calcium ion binding affinities for WT and mutant calmodulins.
