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. 2013 Dec;27(12):4768–4775. doi: 10.1096/fj.13-233965

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Figure 4. — Characterization of CD-1 mice inoculated with rPrP-res^17kDa and rPrP-res^14kDa. A) HE and GFAP staining of mouse brains i.c. inoculated with rPrP-res^17kDa or control inoculum (PBS + BSA) as indicated. B) PrP^Sc in rPrP-res^17kDa-inoculated mouse brain homogenates was detected by PK digestion and immunoblot analysis with 8H4 antibody. Each number represents an individual inoculated mouse. C = undigested mouse brain homogenate was used as a control. C1 and C2 are brain homogenates prepared from mice intracerebrally inoculated with control inocula, inoculum diluent (PBS+BSA) and sPMCA substrate, respectively. C) HE and GFAP staining of mouse brains intracerebrally inoculated with rPrP-res^14kDa or control inoculum (PBS+BSA) as indicated. D) Brain homogenates (BH) prepared from rPrP-res^14kDa-inoculated mice (14-kDa BH) and rPrP-res^17kDa-inoculated mice (17-kDa BH) were subjected to PK digestion and immunoblot analysis with 8H4 antibody. C, control (undigested mouse brain homogenates). E) PK-resistant PrP^Sc in the brain homogenates prepared from mice inoculated with rPrP-res^17kDa (17-kDa BH) and rPrP-res^14kDa (14-kDa BH) was detected by immunoblot analysis with M20 antibody.