Table 2.
Summary of infectivity bioassay in wild-type CD-1 mice
| Inoculum | Diseased/inoculated mice | Survival (dpi) | Average survival (d) |
|---|---|---|---|
| rPrP-res17kDa | 10/10 | 161, 162, 166, 170, 171, 173, 176, 177, 182, 185 | 172.3 ± 1.6 |
| Control 1: sPMCA substrate | 0/5 | >300 | NA |
| Control 2: PBS + BSA | 0/5 | >300 | NA |
| 2nd passage of rPrP-res17kDa: 1% brain homogenates from mice inoculated with i.c. rPrP-res17kDa | 7/7 | 146, 158, 161, 160, 170, 170, 174 | 161.3 ± 1.8 |
| 2nd passage of control mice: 1% brain homogenates from mice inoculated with sPMCA substrate | 0/5 | >250 | NA |
| rPrP-res14kDa | 0/9 | >365 | NA |
| Control: PBS + BSA | 0/5 | >365 | NA |
Average survival values are means ± se. NA, not applicable.