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Effects of HNGF6A are associated with increase in intracellular calcium and are KATP-channel independent. A) Intracellular calcium levels in βTC3 cells grown in 16 mM glucose and treated with or without HNGF6A at 30, 60, and 120 min. B) Effects of HNGF6A on insulin section in the presence of 400 μM diazoxide, a potassium channel activator. Experiments were repeated ≥3 times for calcium (n=16 each) and twice for diazoxide (n=16 each); data are presented as means ± sem. *P < 0.05.
