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. 2013 Dec;27(12):4975–4986. doi: 10.1096/fj.13-235176

Figure 5.

Figure 5.

Rottlerin attenuates peribronchial and perivascular inflammation and MCh-induced AHR in an HDM model of asthma. A) Protocol for asthma induction and rottlerin administration using the HDM model. B) Graph of airway resistance vs. MCh concentration performed on d 22 following initiation of HDM protocol (n=9–12 mice/group). Data are expressed as means ± se. *P < 0.05, ***P < 0.001 for HDM + PBS vs. HDM + rottlerin and control + PBS. C) Lungs from control and HDM-sensitized and challenged mice were H&E stained and photographed using a light microscope. Images are representative of similar results from 4 mice/experimental condition. Scale bars = 100 μm; inset: 50 μm. D) Graph of total cell count and differential of BAL fluid in the 4 treatment groups. Data are expressed as means ± se. **P < 0.01, ***P < 0.001 for HDM + PBS vs. HDM + rottlerin. E) Representative Western blot from whole-lung homogenates of nonsensitized and HDM-sensitized mice treated with either PBS or rottlerin. F) Densitometry analysis of Western blots in E. Data are expressed as means + se. *P < 0.05, HDM + PBS vs. HDM + rottlerin; n = 3–4 mice/group.