Figure 7.

Rottlerin reduces agonist-induced Ca²⁺ oscillations in ASM cells. A) Representative fluorescence confocal image of part of an airway in a lung slice showing epithelial cells (EPCs) lining the airway lumen, underlying smooth muscle cells (SMCs) and a selected 7- × 7-pixel ROI within a SMC for the measurements of the changes in Oregon green fluorescence plotted in B. B) Ca²⁺ oscillations induced by the continuous presence of 100 nM ACh were not altered with the addition of DMSO vehicle control. C) Ca²⁺ oscillations induced by 100 nM ACh were inhibited by 5 μM rottlerin in the continuous presence of ACh. Representative data from 5 experiments using 3 mice.