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. 2013 Dec;27(12):5072–5082. doi: 10.1096/fj.13-238279

Figure 5.

Figure 5.

Cellular stress in cVAChT cardiomyocytes. A) Expression of the cardiac stress markers, β-MHC and ANP, in control and cVAChT cardiomyocytes (n=number of mice). B, C) Immunostaining for ANP in adult cardiomyocytes from control and cVAChT mice. D) Measurement of ROS levels in isolated cardiomyocytes loaded with the MitoSOX superoxide indicator (n=number of cells; ≥3 mice/genotype). E) Measurement of oxidized protein levels in whole hearts from control and cVAChT mice (n=number of mice). F, G) Assessment of calcium transients in isolated ventricular myocytes from VAChTflox/flox and cVAChT mice. F) Representative recordings of line-scan profile of Ca2+ transients in control and cVAChT myocytes. G) Summary of peak Ca2+ (n=number of cardiomyocytes; cells isolated from ≥3 mice/genotype). Scale bars = 25 μm. Data are represented as means ± sem. *P < 0.05 vs. control.