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. 2013 Aug 20;41(21):9753–9763. doi: 10.1093/nar/gkt666

Figure 5.

Figure 5.

CXCL12 and SIKE1 are direct targets of miR-146a-5p. (A) The relative expression levels of CXCL12 and SIKE1 in both MSC types were detected by RT-qPCR (n = 4). (B) CXCL12 and SIKE1 mRNA levels in WJ-MSC transfected with miR-146a-5p antagomirs were analyzed by RT-qPCR. Results are shown as mean ± SD from two duplicates. *P < 0.05 by t-test. (C and D) Putative miR-146a-5p target sites in the CXCL12-3′ UTR (C) and SIKE1-3′ UTR (D). UTR: untranslated region; WT: wild type; MUT: mutant. (E and F) Luciferase reporter assays using the CXCL12-3′ UTR (E) or the SIKE1-3′ UTR (F) in 293T cells transfected with control (Vec) or miR-146a-5p expression vector. Results are presented as mean ± SD of three independent experiments.