Skip to main content
. 2013 Aug 28;41(21):9688–9704. doi: 10.1093/nar/gkt680

Figure 7.

Figure 7.

Confirmation of miR-17-5p and miR-17-3p targeting TIMP3. (a) DU145 cells transfected with RNA mimics (both miR-17-5p and/or miR-17-3p) or a control oligo were subjected to western blot analysis. Cells transfected with RNA mimics (both miR-17-5p and/or miR-17-3p) repressed TIMP3 expression. (b) In invasion analysis, cells transfected with both miR-17-5p and/or miR-17-3p RNA mimics promoted cell invasion. (c) The miR-17-transfected (left) and vector-transfected (right) DU145 cells were transiently transfected with RNA inhibitors (both miR-17-5p inhibitor and/or miR-17-3p inhibitor) or a control oligo. Cell lysates were subject to western blot analysis. Cells transfected with RNA inhibitor promoted TIMP3 expression. (d) The cells were also subject to invasion analysis, both types of cells transfected with miR-17-5p and/or miR-17-3p RNA inhibitor inhibited invasion.