Figure 6.

Med17 inactivation has no effect on start site selection at xMET17 and xhis3 promoters. Primer extension analysis was performed using the same RNA preparations as shown in Figure 4 (xMET17 start sites) and Figure 5 (xhis3 start sites) with a primer starting at position +74 of GFP. The sequencing DNA ladder was generated using the same primer and a plasmid bearing xMET17-GFP or xhis3-GFP. The asterisks indicate the major TSSs. Positions are given relative to the start codon. U3 snoRNA was used as a control.