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. 2013 Aug 19;41(21):9634–9650. doi: 10.1093/nar/gkt725

Figure 4.

Figure 4.

Effect of R-5′-Me-DNA and S-5′-Me-DNA on allele selectivity is dependent on position of incorporation and on the absolute configuration of the 5′-methyl group. Dose-dependent reduction of muHTT and wtHTT mRNA in human GM04022 fibroblasts for (A) ASOs A17 (R-5′-Me-DNA at position 5) and A18 (S-5′-Me-DNA at position 5) and (B) ASOs A19 (R-5′-Me-DNA at position 6) and A20 (S-5′-Me-DNA at position 6). Summary of sequence, activity and selectivity for (C) ASOs A1, A17 and A18 (D) ASOs A1, A19 and A20. Underlined nucleotide indicates position across from the SNP site. Error bars are in ±SD.