Figure 6.

Influence of the glutamine/lysine of the β₂-strand of the DBDs of AtWRKY50 DBD and AtWRKY11 DBD on the DNA-binding specificities. (A) Crude E. coli protein extracts of WRKY11 DBD, 11_DBDQ29K, 50 DBD and 50 DBD_Q26K were analyzed by western blot analyses to show comparable protein concentrations and appropriate protein sizes. (B) Equal amounts of protein extract [3 µg/well] were used to probe dsDNA probes [2 pmol/well] with different versions of the W-box by quantitative DPI-ELISA. The forward sequences of the dsDNA probes are given below the graph. The invariable GAC-core is shaded in gray. The absorbance values were normalized to the mean of the background controls. The absolute error is shown.