Table 2.
Kinetic parameters for canonical DNA cleavage
| Enzyme | KM (nM) | kcat (s−1) | kcat/KM (s−1 M−1) |
|---|---|---|---|
| (×106) | |||
| WT | 17.4 | 0.22 | 12.64 ± 0.4 |
| D16N | 56.9 | 1.21 | 21.26 ± 0.2 |
| D148E | 9.0 | 0.02 | 3.22 ± 0.6 |
| D16N/D148E | 66.9 | 1.09 | 16.31 ± 0.6 |
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