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. 2013 Aug 16;41(21):9881–9890. doi: 10.1093/nar/gkt739

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Relative flexibility of base-pairing mismatches. (A) FRET efficiency as a function of target strand length for target strands containing each of the eight possible single base-pairing mismatches. Each mismatch was probed in molecular vises with five loop sizes (colors as in Figure 1C). The bending rigidity ranked: {intact, GG, GA} > {GT, AA} > {CT, CA, TT, CC}. (B) Flexibility of the mismatches, as quantified by , correlated significantly with the free energy penalty of the mismatch (35) (Spearman’s ρ = 0.90; P = 2.2 × 10⁻³).

Inline graphic — Relative flexibility of base-pairing mismatches. (A) FRET efficiency as a function of target strand length for target strands containing each of the eight possible single base-pairing mismatches. Each mismatch was probed in molecular vises with five loop sizes (colors as in Figure 1C). The bending rigidity ranked: {intact, GG, GA} > {GT, AA} > {CT, CA, TT, CC}. (B) Flexibility of the mismatches, as quantified by , correlated significantly with the free energy penalty of the mismatch (35) (Spearman’s ρ = 0.90; P = 2.2 × 10⁻³).