Figure 2.
Amino acid activation properties of CaLeuRS. Activation of Leu (black square), ABA (black up-pointing triangle), Nva (black down-pointing triangle), Val (black diamond), Ile (white circle), Met (white square) and Ser (white up-pointing triangle) by CaLeuRS. Leu and non-cognate amino acids were used at final concentrations of 1 and 50 mM, respectively. A control reaction without any amino acids (black circle) was included. The insert compares the activation of ABA and the control.