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. 2013 Aug 22;41(21):9825–9838. doi: 10.1093/nar/gkt741

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Recognition of CatRNA^Ser by representative bacterial, yeast, human and archaeal LeuRSs. (A) A representative graph showing aminoacylation of [³²P]CatRNA^Leu with Leu by various LeuRSs. The generated Leu-[³²P]CatRNA^Leu or free [³²P]CatRNA^Leu was separated by TLC. Known amounts of [α-³²P]ATP were serially diluted and loaded onto the TLC plate after separation for quantification. (B) Quantitative analysis of Leu-[³²P]CatRNA^Ser generated by CaLeuRS (black circle), CaLeuRS-D422A (black square), ScLeuRS (black up-pointing triangle) and hcLeuRS (black down-pointing triangle). No charged [³²P]CatRNA^Ser was catalyzed by CamtLeuRS, EcLeuRS and PhLeuRS.