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Aminoacylation kinetic parameters of CaLeuRS-Ser919 and CaLeuRS-Leu919 for the CatRNALeu transcripta
| Enzyme | Km (μM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Relative kcat/Km |
|---|---|---|---|---|
| CaLeuRS-Ser919 | 1.87 ± 0.23 | 0.31 ± 0.05 | 165.78 | 1.0 |
| CaLeuRS-Leu919 | 2.91 ± 0.37 | 0.62 ± 0.08 | 213.06 | 1.3 |
aThe results are the average of three independent repeats with standard deviations indicated.
