Table 2.
Aminoacylation kinetic parameters of CaLeuRS and ScLeuRS for various tRNAsa
| Enzyme | tRNA | Km (μM) | kcat (s−1) | kcat/Km (s−1 mM−1) |
|---|---|---|---|---|
| CaLeuRS | OEb-EctRNALeu | 0.74 ± 0.08 | 0.474 ± 0.026 | 640.54 |
| TSc-EctRNALeu | 2.68 ± 0.39 | 0.555 ± 0.061 | 207.09 | |
| yeast total tRNA | 0.39 ± 0.05 | 0.174 ± 0.019 | 446.15 | |
| OE-hctRNALeu | 0.43 ± 0.05 | 0.639 ± 0.055 | 1486.05 | |
| TS-hctRNALeu | 1.30 ± 0.23 | 0.719 ± 0.094 | 553.08 | |
| ScLeuRS | OE-EctRNALeu | 2.19 ± 0.47 | 2.09 ± 0.16 | 954.34 |
| TS-EctRNALeu | 1.71 ± 0.22 | 0.134 ± 0.011 | 78.36 | |
| yeast total tRNA | 0.332 ± 0.037 | 0.188 ± 0.025 | 566.27 | |
| OE-hctRNALeu | 0.111 ± 0.027 | 2.19 ± 0.22 | 19 729.73 | |
| TS-hctRNALeu | 0.926 ± 0.170 | 0.887 ± 0.114 | 957.88 |
aThe results are the average of three independent repeats with standard deviations indicated.
bOE, over-expressed.
cTS, transcribed.