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kobs values of ScLeuRSs for editing Nva with various tRNAs
| Enzyme | tRNA | kobs (s−1)a |
|---|---|---|
| ScLeuRS | no tRNA | 0.10 ± 0.02 |
| TSb-EctRNALeu | 0.10 ± 0.02 | |
| OEc-EctRNALeu | 0.12 ± 0.03 | |
| CatRNALeu | 0.10 ± 0.03 | |
| TS-hctRNALeu | 0.10 ± 0.01 | |
| OE-hctRNALeu | 0.64 ± 0.04 | |
| ScLeuRS-D419A | no tRNA | 0.094 ± 0.001 |
| OE-hctRNALeu | 0.100 ± 0.010 |
aThe results are the average of three independent repeats with standard deviations indicated.
bTS, transcribed.
cOE, over-expressed.
