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. 2013 Aug 23;41(21):9934–9944. doi: 10.1093/nar/gkt759

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Sedimentation velocity analytical ultracentrifugation. Absorbance c(s) profiles for wild-type, E51A and R83A DdrB at loading concentrations of 20 and 80 µM. Similar profiles were obtained using the interference optical detection system. In addition similar profiles were observed for wild-type DdrB at 0.32 and 0.64 mM. A sedimentation coefficient of 5.76 S was obtained for the wild-type DdrB pentamer in a linear extrapolation of the sedimentation coefficient to zero concentration. The best-fit molar mass for this species was 118 ± 6 kDa, consistent with a DdrB pentamer. Values obtained for the E51A pentamer were 5.64 S and 116 ± 6 kDa, values for the R83A pentamer were 5.57 S and 111 ± 5 kDa. Insets expand the c(s) profiles to highlight contributions from the decamer (∼9.1 S), penta-decamer (∼11.8 S), didecamer (∼14.6 S) and higher species.