Figure 5.

Electrophoretic shift assay of 50b ssDNA by wild-type and E51A DdrB. DNA (10 µM) was incubated with increasing concentrations of DdrB pentamer (0, 2, 10, 20, 50, 100 µM) and resolved on a 4–20% native polyacrylamide gel. Shifts corresponding to DNA-pentamer, DNA-decamer and DNA-pentadecamer nucleoprotein complexes are visible with wild-type DdrB. While the E51A mutant readily forms the DNA-pentamer complex, no distinct shifts corresponding to higher-order assemblies are visible in the gel.